ABH

OrderNo#. 852-05-01

100 mg $138.00

p-Azidobenzoyl Hydrazide

Photoactivatable, carbohydrate-reactive cross-linker

Off white solid. Reacts with cis-diol containing carbohydrates or

other molecules having been oxidized to form aldehydes.

Photoazide end reacts nonspecifically with molecules upon UV

photolysis. Protect from light.

Purity >90% by HPLC. Soluble in methylene chloride.

M.W. 177.17 C7H7ON5

Ref: O’Shannessy, D.J., et al. (1985) J. Applied Biochem. 7, 347-355.

 

ASBA

OrderNo#. 852-05-16

100 mg $90.00

4-(p-Azidosalicylamido) butylamine

Iodinatable and Photoreactive cross-linker

The amine terminal will react with a carboxylic acid in the pressence

of EDC to form an amide bond. Amide product is then ready to

iodinate, then undergo photo-coupling. Off white solid.

Protect from light. M.W. 249.27 C11H15O2N5

BAC

OrderNo#. 882-01-05

5 gm $90.00

N,N,Bis-acryloyl cystamine

Reversible cross-linker for polyacrylamide gels

BAC is used as the cross-linking agent in formulations

of polyacrylamide gels. The resultant cross-linked gel

can be dissolved with 2-mercaptoethenol after electro-

phoresis. m.p. 121-123°. M.W. 260.4 C10H16N2O2S2

Ref: Hansen, J.N. (1976) Anal. Biochem. 76, 37-44.

 

 

 

 

 

BSOCOES

OrderNo#. 852-03-06

100 mg $69.00

Bis-[2-(succinimidooxycarbonyloxy)ethyl] sulfane

Non cleavable, amino reactive, water soluble cross-linker

Off white solid. Base-cleavable, cross-linker useful for studying

receptors and mapping surface polypeptic antigens on

lymphocytes.

M.W. 640.5 C14H16O12N2S

Ref: Howard, A.D., et al. (1985) J. Biol. Chem. 260, 10833.

 

BSSS

OrderNo#. 852-03-01

100 mg $90.00

Bis(sulfosuccinimidyl) Suberate

Non cleavable, amine reactive, water soluble cross-linker

Off white solid. non-cleavable, membrane- impermeable

cross-linker. Used for conjugating radio-labeled ligands to cell

surface receptors. Soluble in H2O. Purity >90% by HPLC.

M.W. 572.4. C16H18O14N2S2Na2

Ref: Staros, J.V. (1982) Biochemistry 21, 3950.

 

 

DMAI

OrderNo#.852-04-00

10 gm $49.00

Dimethyl Adipimidate.2HCl

Homobifunctional Imidoester Cross-linker

White powder. Used to cross-link outer membrane proteins.

Purity >95% by titation. M.W. 245.1

CAS14620-72-5 C8H16N2O2.2HCl

Ref: Wang, D. and Moore, S. (1977) Biochemistry 16, 2937-2941.

 

 

DMPI

OrderNo#. 852-04-01

10 gm $76.00

Dimethyl Pimelimidate.2HCl

Homobifunctional Imidoester Cross-linker

White solid. Water soluble homobifunctional cross-linker that

reacts rapidly with amines at pH 8-10. The amide bond formed

retains net change character of the protein. Purity >95% by

tritation. M.W. 259.2 CAS 58537-94-3 C9H18N2O2.2HCl

Ref: Brew, K., et al. (1975) J. Biol. Chem. 250, 1434-1444.

 

 

DMSI

OrderNo#. 852-04-02

10 gm $76.00

Dimethyl Suberimidate.2HCl

Homobifunctional Imidoester Cross-linker

White solid. Water soluble homobifunctional cross-linker that

reacts rapidly with amines at pH 8-10. The amide bond formed

retains net change character of the protein. Purity >95% by

tritation. M.W. 273.2 CAS 34490-86-3 C10H20N2O2.2HCl

Ref: Dower, S.K., et al. (1981) Biochemistry 20, 6326.

 

DTBP

OrderNo#. 852-04-03

1 gm $58.00

Dimethyl 3,3-dithiobispropionimidate.2HCl

Cleavable Imidoester Cross-linker

White solid. Penetrates intact human erythrocytes and cross-links

many of the membrane proteins to hemoglobin. M.W. 309.3

CAS 38285-78-8 C8H16N2O2S2.2HCl

Ref: Wang, K. and Richards, F.M. (1974) J. Biol. Chem. 249, 8005-8018.

 

 

HSAB

OrderNo#. 852-05-06

100 mg $52.00

N-Hydroxysuccinimidyl-4-azidobenzoate

Photoaffinity Cross-linker

White solid. HSAB is ideal for a wide range of photoaffinity

applications. Light sensitive. Purity 95%.

M.W. 260.2 CAS 53053-08-0 C11H8N4O4

Ref: Galardy, R.E., et al., (1974) J. Biol. Chem. 249, 3518.

 

 

MBS

OrderNo#. 852-02-02

100 mg $49.00

m-Maleimidobenzoyl-N-hydroxysuccinimide ester

Heterobifunctional Cross-linker

White solid. A cross-linker that is useful for conjugation of

enzymes to antibodies, haptens to carrier proteins and toxins

to antibodies. Purity >95% by TLC. Soluble in DMSO and Chloroform.

M.W. 314.2 CAS 58626-38-3 C15H10N2O6

Ref: Kitagawa, T. and Aikawa, T.J., (1976) Biochem. 79, 233.

 

 

NHS-ASA

OrderNo#. 852-05-03

100 mg $69.00

N-Hydroxysuccinimidyl-4-azidosalicylic acid

Photoreactive, Iodinatable Cross-linker

Solid. NHS ASA can be radiolabled before the acylation

step. The photolysis reaction can be easily initiated by

long wave UV light. Light sensitive.

M.W. 276.21 C11H8N4O5

Ref: Ji, T.H. and Ji, I., (1982) Anal. Biochem 121, 286.

 

 

PIP

OrderNo#. 852-01-00

10 gm $76.00

100 gm $621.00

Diacrylyl piperazine

Electrophoresis Cross-linker

White solid. Pip is a unique polyacrylamide gel cross-linker.

Used in place of Bis N,N-methylene-bis-acrylamide. Pip provides

increased gel strength, improved electrophoretic seperation of

proteins and less background during silver staining. Purity >99%.

M.W. 194.2 CAS 6342-17-2 C10H14N2O2

Ref: Patchornik, A., et al. (1988) Anal. Biochem. 173, 412.

 

SULFO HSAB

OrderNo#. 852-05-07

100 mg $69.00

N-Hydroxysulfosuccinimidyl-4-azidobenzoate

Photoreactive Cross-linker

Off white solid. Sulfo HSAB is ideal for a wide range of photoaffinity

applications. Light sensitive. M.W. 362.25 C11H7N4O7Na

Ref: Galardy, R.E., et al. (1974) J. Biol. Chem. 249, 3510-3518.

 

SULFO SADP

OrderNo#. 852-05-12

100 mg $138.00

N-Succinimidyl)4-azidophenyl)1,3’-dithiopropionate

Photoreactive Cross-linker

Sulfo SADP is cleavable by 50 mM dithiothreitol,

100 mM mercaptoethanol, or 1% sodium borohydride.

Photolysis is achieved by irradiation at 267-275 nm.

Water soluble. Light sensitive. M.W. 454.45

C13H11N4O7SNa

Ref: Zarling, D.A., et al. (1982) J. Immunol. 128, 251-257.

 

 

SULFO SAMCA

OrderNo#. 852-05-14

50 mg $138.00

Sulfosuccinimidyl-7-azido-4-methylcoumarin-3-

acetate

Fluorescent Cross-linker

An amine reactive and photoreactive cross-linker. It is non-cleavable

and has fluorescent features. The cross-linked product will contain

the fluorescent modified AMCA. Light sensitive. M.W. 458.34

 

SULFO SANPAH

OrderNo#. 852-05-09

100 mg $138.00

Sulfosuccinimidyl6-(4-azido-2-nitrophenylamino)

hexanoate

Photoreactive Cross-linker

Orange-red solid. Photolysis of sulfo-SANPAH occurs at

320-350 nm. This limits damage to biomolecules by irradiation.

This compound is a water soluble, extended chain length analog

to ANB-NOS. Light sensitive. Water soluble. M.W. 492.39

C16H17N6O9SNa

Ref: Schmitt, M., et al. (1983) J. Biol. Chem. 258, 649-654.