N-[4-(p-Azidosalicylamido)butyl]-3-(2-pyridyldithio)Propionamide

Sulfhydryl-photoreactive reagent

A cleavable, sulfhydryl reactive photo reactive cross-linker that is

radioiodinatable. Off white solid. Protect from light.

M.W. 446.55 C₁₉H₂₁O₃N₆S₂

Ref: Traut, R.R., et al. Protein function, A Practical Approach.

Edited by T.E. Creighton, IRL Press, 1989, p. 101.

Bis[B-(4-azidosalicylamido)ethyl] disulfide

Cleavable photoreactive reagent that is iodinatable

BASED reacts nonspecifically with proteins. It is useful when no

amines or sulfhydryls are in a location suitable to obtain the

desired conjugate. Protect from light. Off white solid.

Purity >90% by elemental analysis.

M.W. 474.54 C₁₈H₁₈O₄N₈S₂

N-[6-(Biotinamido)hexyl]-3^’-(2^’-pyridyldithio) Propionamide

Sulfhydryl Reactive Biotinylation Reagent

Biotinylation occurs between pH 6-9. Pyridyldithiol reacts with

sulfhydryl groups to form a stable disulfide bond. The

pyridine-2-thione leaving group can be measured at 343 nm to

determine the degree of biotinylation. White solid.

Purity >90% by HPLC. M.W. 539.77 C₂₄H₂₇O₃N₅S₃

Ref: Ghebrehiwet, B., et al. (1988) J. Immunol. Methods 110, 251-260.

Carbohydrate Reactive Biotinylation Reagent

Biotinylates immunoglobulins without loss of immunological

activity. Use at pH 4-6 to complete coupling reaction. White powder.

Purity >95% by TCL. CAS 66640-86-6.

M.W. 258.3 C₁₀H₁₈O₂N₄S

Ref: O’Shannessy, D.J., et al. (1984) Immun. Letters 8, 273-277.

Biotinamidocaproyl Hydrazide

Carbohydrate Reactive, Spacer Arm, Biotinylation Reagent

Biotinylates oxidized sugars, glycoproteins with a 6 carbon spacer

between biotin and the ligand, alleviating steric hindrance.

Off white solid. Purity >95%. Soluble in MeOH and H₂O.

M.W. 371.5 C₁₆H₂₉O₃N₅S

Ref: O’Shannessy, D.J. et al. (1987) Anal. Biochem. 163, 204-209.

Bismaleimido butane

Homobifunctional Reagent

White solid. Used as a bridge between free sulfhydryl

groups of biological molecules. The two maleimido functional

groups permit irreversible cross-linking of sulfhydryl containing

compounds under mild conditions.

M.W. 248.29 C₁₂H₁₂N₂O₄

Bismaleimido hexane

Homobifunctional Reagent

White solid. Used as a bridge between free sulfhydryl

groups of biological molecules. The two maleimido functional

groups permit irreversible cross-linking of sulfhydryl containing

compounds under mild conditions.

M.W. 276.29 C₁₄H₁₆N₂O₄

Bismaleimido propane

Homobifunctional Reagent

White solid. Used as a bridge between free sulfhydryl groups

of biological molecules. The two maleimido functional groups

permit irreversible cross-linking of sulfhydryl containing com-

pounds under mild conditions.

M.W. 234.29 C₁₁H₁₀N₂O₄

(1-biotinamido)-4-[4-(maleimidomethyl) cyclohexane-

carboxamido] butane

Sulfhydryl Reactive Biotinylation Reagent

Biotin maleimido reagent that reacts at a neutral to slightly acidic

pH to form a thiol ether linkage. White solid. Soluble in DMF.

M.W. 561.7 C₂₈H₄₃O₅N₅S

N-Succinimidyl-3-(4-hydroxyphenyl) propionate

Iodination Reagent

Reacts with N-terminal amino groups at pH 8.5. Iodinate before

or after coupling to molecule of interest. White solid. M.W. 263.26

Purity: 90%. CAS 3407-95-9 C₁₃H₁₃O₅N

Ref: Bolton, A.E. and Hunter, W.M. (1973) Biochem. J. 133, 529-539.

Sulfosuccinimidyl-3-(4-hyroxyphenyl) propionate

Water Soluble Iodination Reagent

Reacts with N-terminal amino groups at pH 8.5. Iodinate before

or after coupling to molecule of interest. White solid. M.W. 365.30

C₁₃H₁₂O₈NSNa

Ref: Thompson, J.A., et al (1987) Biochem 26, 743-750.

G.C. Silylation Reagent

Forms highly stable TMS derivatives wtih most organic functional

groups under mild reaction conditions. Purity >97%. M.W. 203.43.

CAS 10416-59-8 C₈H₂₁NOSi₂

Ref: Welch, M.J., et al. (1993) J. of Toxicology 17, 389.

100 gm $138.00

G.C. Silylation Reagent

Reacts faster than BSA, due to presence of TFA group.

Biproducts and BSTFA are highly volatile allowing good

seperation of early eluding peaks. Purity >97%.

M.W. 257.4 CAS 25561-30-2 C₈H₁₈ONSi₂F₃

Ref: Wang, W.L., et al. (1994) J. of Chrom. B 660, 279

G.C. Silylation Reagent

Addition of TMCS to BSTFA catalyzes reactions of hindered

functional groups in secondary alcohols and amines.

Purity >97%. CAS 25561-30-2.

Ref: Cone, E.J., et al. (1994) Clinical Chemistry, 40 (7), 1299.

Bis(1,1-trifluoroacetoxy) iodobenzene

Protein Modification Reagent

Used for estimating asparagine and glutamine in peptides and

proteins. Has also been used to determine selenocyanate

and thiocyanate ions in the presence of cyanide.

Ref: Soby, L.M. and Johnson, P. (1981) Anal. Biochem. 113, 149.

4-N-N-Dimethylaminoazobenzene 4-Isothiocyanate

Amino Acid Derivatizing Reagent

Red-orange solid. Purity >90% by TLC. M.W. 282.4

CAS 7612-98-8 C₁₅H₁₄N₄S

Ref: Chang, J.Y. and Creaser, E.H., et al. (1976) Biochem. J. 153, 607.

5-Dimethylaminonapthalene-1-sulfonyl chloride

Fluorometric Detection Reagent

Yellow-orange solid. Yeilds highly fluorescent peptide

derivatives. Purity .95%. M.W. 269.7

CAS605-65-2 C₁₂H₁₂NO₂SCl

Ref: Oray, B., et al. (1983) J. Chromatogr. 270, 253.

p-phenylene diisothiocyanate

Homobifunctional Reagent

Crystalline solid. Used to couple peptides containing lysine

to amino polystyrene resins. Soluble in CHCl₃ and DMF.

M.W. 192.3. CAS 4044-65-9. C₈H₄N₂S₂

Ref: Laursen, R.A., et al. F E B S Lett. 21, 67 (1972).

1,4-Di[3’-(2’-pyridyldithio)propionamido]butane

Sulfydryl Reactive Homobifunctional Reagent

White solid. Reacts with sulfhydryls to form a mixed disulfide.

The disulfide can be readily cleaved with a reducing reagent.

Purity >90% by HPLC. M.W. 482.7 C₂₀H₂₆N₂O₂S₄

Dithiobis (succinimidyl propionate)

Cleavable Homobifunctional Reagent

White solid. A thiol cleavable cross-lilnking reagent. 50 mMDTT,

100 mM mercaptoethanol or 1% sodium borohydride can be used

to reduce the disulfide bond. M.W. 404.4 CAS 57757-57-0

C₁₄H₁₆N₂O₈S₂

Ref: Tavers, R.C., et al. (1982) J. Biol. Chem. 257, 10708.

Disuccinimidyl Suberate

Homobifunctional Reagent

White solid. Noncleavable, amine reactive homobifunctional

cross-linker. Has been used for conjugating radiolabeled ligand

to cell surface receptor. Purtiy >95% by TLC. M.W. 368.3

CAS 68528-80-3 C₁₆H₂₀N₂O₈

Ref: Sawyer, S.T., et al. (1987) PNAS 84, 3690-3694.

Dissuccinimidyl Tartarate

Cleavable Homobifunctional Reagent

White solid. A cleavable, homobifunctional reagent. Can be

cleaved with .015M sodium periodate or other oxidizing

reagents. M.W. 344.24 C₁₂H₁₂N₂O₁₀

Ref: Smith, R.J., et al. (1978) Biochemistry 17, 3719-3723.

Dithiobis Phenylazide

Photoreactive Homobifunctional Reagent

Photoreactive reagent that is sensitive to subtle changes in

membrane structures as a function of pH. Reversible by disulfide

reduction wtih mercaptoethanol. Light sensitive.

M.W. 300.4 CAS 37434-06-3 C₁₂H₈N₆S₂

Ref: Mikkelsen, R.B. and Wallach, D.F.H. (1976) J. Biol. Chem. 251, 7413.

3,3 Dithiobis (sulfosuccinimidyl propionate)

Homobifunctional Reagent

Off white solid. Thiol cleavable cross-linker. The disulfide bond

can be cleaved with 50 mM DTT, 100 mM mercaptoethanol or

1% NaBH₄. Has been used to immobilize proteins onto supports

containing amino groups. Purity >95% by HPLC. M.W. 608.5

C₁₄H₁₂N₂O₁₄S₄Na₂

Ref: Lee, W.T. and Conrad, D.H. (1985) J. Immunol. 134, 518-525.

Ethyleneglycol-bis-(succinimidylsuccinate)

Homobifuctional Reagent

White solid. Used for cross-linking and reversible immobilization

of proteins through their amine groups. This reagent has been

used to study structure-function relationships. Readily cleaved at

pH 8.5 with hydroxylamine for 3-5 hours at 37°C. Purity >80% by

HPLC. Soluble in DMSO. M.W. 456.4 CAS 70539-42-3

C₁₈H₂₀N₂O₁₂.

Ref: Conn, P.M., et al. (1982) Nature 296, 653-655.

Maleimido caproic acid hydrazide^.HCl

Heterobifunctional Reagent

White solid. Has an oxidized carbohydrate specific hydrazide

and a sulfhydryl reactive maleimide. Spacer arm accommodates

a wide range of molecular cross-linking demands.

M.W. 261.8 C₁₀H₁₅N₃O₃^.HCl

Maleimido caproic acid N-hydroxysuccinimide ester

Heterobifunctional Reagent

White solid. Sulfhydryl and amine reactive reagent used for

enzyme immunoassay. M.W. 308.3 CAS 55750-63-5

C₁₄H₁₆N₂O₆

Ref: Fujiwara, K., et al. (1981) J. Immunol. Meth., 45, 195.

Maleimidobutyric acid N^.hydoxysuccinimide Ester

Heterobifunctional Reagent

White solid. Useful for preparation of conjugates for EIA procedures

or for immobilizing enzymes on solid supports. Has a low potential

for illiciting an immune response. M.W. 280.2 C₁₂H₁₂N₂O₆

Ref: Rusin, K.M., et al. (1992) Biosens. Bioelectron. 7, 367.

G.C. Acylation Reagent

Colorless liquid. Ideal for preparing fluoroacyl derivitives for GC/MS.

HFAA derivitives are the most sensitive to ECD. Purity >99%.

M.W. 410.06 CAS 336-59-4 C₈O₃F₁₄

Ref: Jones, J.B., et al. (1993) J. of Analytical Toxicology 17, 447.

Heptafluorobutyryl Imidazole

G.C. Acylation Reagent

Colorless liquid. Undergoes fast reactives with phenols,

alcohols and amines to form derivatives that are suitable

for ECD. The imidazole by product is not acidic so no

decomposition or corrosion occurs on the column.

Purity >99%. M.W. 264.1 C₅H₃N₂OF₃

Ref: Miyazaki, H., et al. (1973) Anal. Chem. 45 (7) 1164.

1,1,1,3,3,3-Hexamethyldisilazane

G.C. Silylation Reagent

Colorless liquid. HMDS is a weak TMS doner that is used for

silylation of carbohydrates. Purity >97%. M.W. 161.4

CAS 999-97-3 C₆H₁₉NSi₂

Ref: Swealey, C.C., et al (1963) JACS 85, 2495-2507.

p-Hydroxyphenyl Glyoxal

Protein Modification Reagent

Solid. Reactsspecifically with arginine residues at pH 7-9 at

25°C. Reaction can be monitored at 340 nm at pH 9.

M.W. 168.15 C₈H₆O₃^.H₂O

Ref: Yamasaki, R.B., et al, (1980) Anal. Biochem. 109, 32-40.

3-(4-hydroxphenyl) propionic acid hydrazide^.HCl

Iodination Reagent

White solid. A hydrazide analog of Bolton-Hunter Reagent, for

coupling to oxidized carbohydrates.

M.W. 216.65 C₉H₁₂N₂O₂^.HCl

N-Iodoacetyl-N-biotinylhexylenediamine

Biotinylation Reagent

Off white solid. Reacts specifically with thiol groups at pH 7.5-8.5

to form a stable thiol-ether bond. Reagent is not water soluble

so a small amount of DMSO or DMF should be added to the ligand

containing buffer. Soluble in DMSO, DMF.

M.W. 510.42 C₁₈H₃₁N₄O₃SI

Ref: Suton, K., et al. (1984) J. Mol. Bio. 178, 323-339.

Protein Modification Reagent

White solid. One step modification reagent for cysteine. Under

alkylation conditions the sulfhydryl group is ethylated and the

trifluoroacetyl group is lost to give an amine. M.W. 267.0

CAS 67680-56-2 C₄H₅NOF₃I

Ref: Schwartz, W.E., Smith, P.K., and Royer G.P. et al(1980) Anal. Biochem. 106, 43-48.

Protein Modification Reagent

White solid. Protein cleavage at the tryptophenyl bond have been

acheived with this reagent while tyrosine and histidine are unaffected.

M.W. 264.0 CAS 304-91-6 C₇H₅O₃I

Ref: Mahoney, W.C., et al. (1981) Biochemistry 20, 443-448.

Enzyme Test Reagent

White solid. IPTG is an inducer of B-D-galactoside. IPTG is used

to induce the expression of cloned genes that are linked to the lac

promotoer, and for selection of lac Y mutants. Purity >90% by TLC.

Soluble in alcohol and H₂O. M.W. 282.4 CAS 367-93-1

C₉H₁₈O₅S

Ref: (1981) J. Immunol. 127, 1652.

Iodination Reagent

Yellow solid. Amine reactive iodination reagent.

M.W. 178.97 C₇H₆NO₂SB

1-Fluoro-2,4-dinitrophenyl-5-l-alanine-amide

Detection Reagent

Yellow solid. Margey’s derivatizes optical isomers in less than

2 hours. Derivatives can be detected by UV at 340 nm with

picomole sensitivity. M.W. 272.2 CAS 95713-52-3

C₉H₉N₄O₅F

Ref: Marfey, P., (1984) Carlsberg Res. Commun. 49, 591.

N-Methyl-bis-(heptafluorobutyramide)

G.C. Acylation Reagent

Colorless liquid. Used in dual derivatization with a silylation

reagent (MSTFA) eliminatng any acidty or oxidation step. Procedes

optimal seperation of amphetamine, methamphetamine,

phenylpropanolamine and ephedrine which also elute in this order.

M.W. 423.1 C₉H₃NO₂F₁₄

Ref: Clouette, J., et al. Abstract 48, SOFT, Utah 1997.

G.C. Acylation Reagent

Colorless liquid. Reacts quickly under mild conditions with primary

and secondary amines. Reacts slower with alcohols, phenols and

thiols. Is also useful in the analysis of sugars. Purity >98%.

M.W. 223. 08 CAS 685-27-8 C₄H₃NO₂F₆

Ref: Donike, M. (1973) J. of Chem. 78, 273.

Maleimidopropionic Acid Hydrazide

Heterobifunctional Reagent

White solid. Has an oxidized carbohydrate specific hydrazide

and a sulfhydryl reactive maleimide. Spacer arm is shorter than

EMCH. M.W. 219.63 C₇H₉N₃O₃^.HCl

Maleimidopropionic acid N-hydroxysuccinimide ester

Heterobifunctional Reagent

White solid. Sulfhydryl and amine reactive reagent used for

enzyme immunoassay. Shorter spacer arm than EMCS.

M.W. 266.2 CAS 55750-62-4 C₁₁H₁₀N₂O₆

Ref: Kitagawa, T., Enz. Immunoassay, 81, (1981).

N-Methyl-N-trimethylsilyl-heptafluorobutyramide

G.C. Silylation Reagent

Colorless liquid. This reagent is a TMS derivatizing reagent that

has an increased fluorine content that causes less SiO₂ fouling

of FID detectors. M.W. 299.26 C₈H₁₂NOF₇Si

Ref: Donike, M. (1976) Chem. Abstr. 85, 103518.

G.C. Silylation Reagent

Colorless liquid. Useful in the analysis of volatile trace materials.

The most volatile of the TMS-acetamides. Purity >97%.

M.W. 199.25 CAS 24589-78-4 C₆H₁₂NOSiF₃

Ref: Gleispuch, H. (1974) j. Chrom. 91, 407-412.

with 1% TMCS

G.C. Silylation Reagent

Colorless liquid. The most volatile TMS-acetamide available.

TMCS helps catalyze reaction. Forms volatile and stable derivatives

for GC/MS.

M.W. 199.25 CAS 24589-78-4 C₆H₁₂NOSiF₃

Ref: Doniker, M. (1969) J. Chromatogr. 42, 103-104.

G.C. Silylation Reagent

Colorless liquid. MTBSTFA derivatives are more stable to hydrolysis

than their corresponding TMS derivatives. Reacts with active

hydrogens to form stable t-BDMS derivatives.

M.W. 241.33 CAS 77377-52-7 C₉H₁₈NOSiF₃

Ref: Harkey, M.R., et al. (1991) J. of Anal. Tox. 15, 260.

with 1% t-butyl DMCS

G.C.Silylation Reagent

Colorless liquid. Same as MTBSTFA but the addition of

1% t-BDMCS helps catalyze reactions of hindered alcohols

and amines. M.W. 241.33 CAS 77377-52-7

C₉H₁₈NOSIF₃

Ref: West, R.E., et al. (1993) J. of Anal. Tox. 17, 114.

N-Hydroxysuccinimido biotin

Biotinylation Reagent

Off white solid. Biotinylates amino acids, peptides or proteins

under mild conditions. Purity >95% by TLC. Soluble in DMSO.

M.W. 341.3 CAS 35013-72-0 C₁₄H₁₉N₃O₅S

Ref: Ngo, T.T., et al. (1982) Applied Biochem. Biotech. 7, 443-454.

N-Hydroxysuccinimidyl Bromoacetate

Heterobifunctional Reagent

White solid. NHS Bromoacetate allows bromoacetylation of

primary amine groups followed by coupling to sulfhydryl-containing

compounds. Protect from light. M.W. 236.0 CAS 42014-51-7

C₆H₆BrNO₄

Ref: Bernatowicz, M.S. and Matsueda, G.R. (1986) Anal. Biochem. 155, 95.

N-Hydoxysuccinimidyl Iodoacetate

Heterobifunctional Reagent

Off white solid. Iodoacetylates primary amine groups followed by

coupling to sulfhydryl containing compounds. Protect from light.

M. W. 283.0 CAS 39028-27-8 C₆H₆NO₄I

Ref: Rector, E.S., et al. (1978) J. Immunol. Methods 24, 321.

Succinimidyl-6-(biotinamido) hexanoate

Biotinylation Reagent

White solid. Used to biotinylate amino acids, peptides or proteins

by reacting with primary amines under mild conditions.

Purity >90% by TLC. Soluble in DMSO. M.W. 454.5

CAS 72040-63-2 C₂₀H₃₀N₄O₆S

Ref: Green. N.M., et al. (1971) Biochem J. 125, 781.

Succinimidyl 6-[3-(2-pyridylthio)propionamide] hexanoate

Heterobifunctional Reagent

Off white solid. SPDP analog with an extended spacer arm. The

extended spacer arm can reduce the effect of steric hinderances.

M.W. 425.52 C₁₈H₂₇N₃O₅S₂

Ref: Carlsson, J., et al. (1978) Biochem. J. 173, 723-737.

o-Phthaldehyde

Amino Acid Detection Reagent

Yellow needles. A specially purified fluorogenic grade, for

detection of amino acids. OPA is highly purified to reduce

background and specially tested to resist oxidation. An intense

blue fluorescence is produced when added to amines. OPA is

often used at a concentration of 0.8mg/ml dissolved in a

potassium borate buffer solution, pH 10.4 containing Brij 35

and 2-mercaptoethanol. Purity >99%. M.W. 134.1

CAS 643-79-8 C₈H₆O₂

Ref: Lee, H. et al. (1979) Anal. Biochem. 96, 298-307.

Enzyme Test Reagent

White solid. Chromogenic substrate for horseradish peroxidase

in ELISA procedures. Protect from light.

M.W. 181.1 CAS 624-18-0 C₆H₈N₂^.2HCl

Ref: Merck Index 12, 7438.

p-Maleimidophenyl isocyanate

Heterobifunctional Reagent

Pale yellow solid. Useful for hydroxyl and thiol cross-linking.

Has been used in the preparation of alkaline phosphatase

conjugates of estradiol, vitamin B₁₂, progesterone and serine

enriched peptides. Hydoscopic. Purity >90%.

Soluble in DMSO and DMF. M.W. 214.2 C₁₁H₆N₂O₃

Ref: Palumbo, P.S., et al. (1993) Bioconjugate Chem. 4, 212-218.

Sulfosuccinimidyl (4-azidosalicylamido) hexanoate

Heterobifunctional Reagent

A spacer arm version of NHS ASA. The spacer arm helps to

overcome steric restraints. Water soluble. Light sensitive.

M.W. 491.41 C₁₇H₁₇N₅O₉SNa

Ref: Vander Horst, G., et al. (1990) J. Biol. Chem. 265 (19), 10801.

Sulfosuccinimidyl 2-(m-azido-o-nitrobenzamido)

ethyl-1,3-dithio-propionate

Heterobifunctional Reagent

Phenylazide with nitro group that allows photolysis at 320-350 nm.

Is cleavable by thiols and is water soluble. Light sensitive.

M.W. 570.52 C₁₆H₁₄N₆O₁₀S₂Na

Ref: Lewis, R.V., et al. (1977) Biochemistry 16, 5650-5654.

Sulfsuccinimidyl-2-[p-azidosalicylamido]

ethyl-1,3’-dithiopropionate

Heterobifunctional Reagent

A cleavable, photoreactive and radiodinatable cross-linker.

Iodination occurs between the azide and hydroxyl group of the

phenyl ring. After cleaving the disulfide bond with a reducing

reagent, the radioactive label will remain with the protein

conjugated by photolysis. Water soluble. Light sensitive.

M.W. 541.51 C₁₆H₁₅N₅O₉S₂Na

Ref: Ji, T.H. and Ji, I. (1982) Anal. Biochem. 121, 286-289.

N-Succinimidyl-S-acetylthioacetate

Protein Modification Reagent

White solid. Used to introduce protected thiol groups onto

primary amines. The protected sulfhydryl prevents dipeptide

formation. When treated with hydroxylamine, yields a free

sulfhydryl group that can be conjugated to maleimide modified

protein. TLC one spot. Soluble in DMSO and methylene chloride.

M.W. 231.2 CAS 76931-93-6 C₈H₉NO₅S

Ref: Duncan, R.J., et al (1983) Anal. Biochem. 132, 68.

N-Succinimidyl (4-iodoacetyl) Aminobenzoate

Heterobifunctional Reagent

Off white solid. Amine and sulfhydryl reactive reagent. Used

to make enzyme-antibody conjugates. M.W. 402.15

C₁₅H₁₁N₂O₅I

Ref: Weltman, J.K. et al. (1983) Bio Techniques 1, 148-152.

Succinimidyl-4-(N-maleimidomethyl)cyclohexane-

1-carboxylate

Heterobifuntional Reagent

White solid. Useful for enzyme labeling of antibodies and anti-

body fragments. Soluble in acetonitrile and DMF. Purity >98%

by TLC. M.W. 334.3 CAS 64987-85-5 C₁₆H₁₈N₂O₆

Ref: Yoshitake, S., et. al (1982) J. Biochem. 56, 56-63.

Succinimidyl 4-(p-maleimidophenyl) Butyrate

Heterobifunctional Reagent

Yellow solid. Conjugates made with SMPB were shown to be more

stable in serum than SPDP conjugates. SMPB is a long chain

analog of MBS. M.W. 356.3 CAS 79886-55-8 C₁₈H₁₆N₂O₆

Ref: Martin, F.J. (1982) J. Biol. Chem. 257, 286-288.

Succinimidyl-3-(2-pyridyldithio) propionate

Heterobifunctional Reagent

White Solid. Used to introduce thiol groups in their protected form

into a variety of proteins. Treatment with DTT releases 2-pyridylthione

and mercaptopropionyl-modified amine. The thiolated molecule

can then be coupled to a second molecule through a halogon

acetamide or maleimide group. The thiolated molecule can also

be reacted with a second pyridyldisulfide containing molecule.

Purity >97%. Soluble in DMF. M.W. 312.4 CAS 68181-17-9

C₁₂H₁₂N₂O₄S₂

Ref: Vallera, D.A., et al. (1997) J. Immunol. Methods 197, 69.

Disulfosuccinimidyl Tartarate

Homobifunctional Reagent

White solid. Sulfo DST is the water soluble analog of DST.

Sulfo DST is periodate cleavable and offers reaction conditions

similar to DST. M.W. 548.34 C₆H₁₀N₂O₁₆S₂Na₂

Ref: Smith R.J., et al. (1978) Biochemistry 17, 3719-3723.

N-(maleimidocaproyloxy)sulfosuccinamide

Heterobifunctional Reagent

White solid. Water soluble analog of EMCS. Used for enzyme

immunoassy. Water soluble. M.W. 410.35 C₁₄H₁₅N₂O₉SNa

Ref: Kitagawa, T., et al. (1981) Chem. Pharm. Bull. 29, 1130.

N-Maleimidobutyryloxysulfosuccinimide ester

Water Soluble Heterobifunctional Reagent

White solid. Useful for preparation of conjugates for EIA

procedures or for immobilizing enzymes on solid supports.

Soluble in H₂O. M.W. 328.24 C₁₂H₁₁N₂O₉SNa

Ref: Fujiwara, K., et al. (1988) J. Immunol. Methods 110, 47.

Sulfosuccinimidyl-6-(biotinamido)hexanoate

Biotinylation Reagent

Off white solid. Readily couples biotin to primary amino groups of

properties under mild alkaline conditions. Labels antibodies,

hormones or other polypeptide molecules containing lysine

M.W. 556.6 C₂₀H₂₉N₄O₉S₂Na

Ref: Green, N.M., et al. (1971) Biochem. J. 125, 781.

Maleimidopropionic acid-N-hydoxysulfosuccinimide ester

Heterobifunctional Reagent

Off white solid. Sulfhydryl and amine reactive reagent used for

enzyme immunoassay. Shorter spacer arm than GMBS.

Water soluble. M.W. 368.19 C₁₁H₉N₂O₉SNa

Ref: Fujiwara, K., et al.(1988) J. Immunol. Methods 110, 47.

N-Hydroxysulfosuccinimide

Protein Modification Reagent

Off white solid. Used to form amine reactive peptides. Adding

sulfo-NHS to carbodiimide mediated coupling reactions results

in an increase of hapten-carrier protein conjugates.

Soluble in H₂O. M.W. 217.14 C₄H₄NO₆SNa

Ref: Staros, J.V., et al. (1986) Anal. Biochem. 156, 220-222.

Sulfosuccinimidyl Acetate

Protein Modification Reagent

Off white solid. A water soluble acylating reagent. Reacts mainly

with primary amine groups at pH 7 or greater. This irreversible

reaction is accompanied by the release of sulfo NHS.

M.W. 259.17 C₆H₆NO₇SNa

N-Hydroxysulfosuccinimido biotin

Biotinylation Reagent

Off white solid. Reacts with primary amines to biotinylate proteins

under mild conditions. Can be used to biotinylate cell surface

proteins and proteins that are sensitive to organic solvents.

Water soluble. M.W. 443.4 C₁₄ H₁₈ N₃ O₈S₂Na

Ref: Ingalls, H.M., et al. ( 1986) PNAS 83, 4779-4783.

Sulfosuccinimidyl6-[3-(2-pyridyldithio)propionate]

Hexanoate

Heterobifunctional Reagent

Off white solid. Long chain water soluble analog of SPDP. The

extended spacer arm can reduce the effect of steric hinderances.

M.W. 527.56 C₁₈H₂₆N₃O₈S₃Na

Ref: Carlsson, J., et al. (1978) Biochem. J. 173, 723-737.

Sulfosuccinimidyl-2-biotinamidoethyl-1,3-dithiopropionate

Cleavable Biotinylation Reagent

Off white solid. A water soluble biotinylation reagent that is

reversible. Useful for extracting non-covalently associated

proteins from complex physiological mixtures under mild

isolation conditions. Soluble in H₂O. M.W. 606.7

Ref: Gratch, D.R., et al. (1987) Anal. Biochem. 163, 270.

Sulfosuccinimidyl-4-o-(4,4-dimethoxytrityl)-butyrate

Amine Detection Reagent

Used to detect amine groups on solid supports. Water soluble.

M.W. 665.6 C₂₉H₂₈NO₁₀SNa

Ref: Gaur, R.K. and Gupta, K.C. (1989) Anal. Biochem. 180, 253-258.

Sulfosuccinimidyl(4-iodoacetyl)aminobenzoate

Heterobifunctional Reagent

Off white solid. A water soluble amine and sulfhydryl reactive

reagent. Used to make enzyme antibody conjugates.

Water soluble. M.W. 504.20 C₁₅H₁₂N₂O₈INa

Ref: Weltman, J.K., et al. (1983) Bio. Techniques 1, 148-152.

Sulfosuccinimidyl 4-(N-maleimidomethyl)

cyclohexane-1-carboxylate

Heterobifunctional Reagent

Off white solid. Ideal for coupling enzymes to antibodies. Sulfo

SMCC contains a cyclohexane bridge, that gives extra stability

to the maleimide group. Enzyme activity and antibody specificity

are preserved after coupling. Water soluble. Purity >90% by HPLC.

M.W. 436.4 CAS 92921-24-9 C₁₆H₁₉N₂O₉SNa

Ref: Hushida, S., et al. (1984) J. Applied Biochem. 56, 56-63.

Sulfosuccinimidyl4-(p-maleimidophenyl)butyrate

Heterobifunctional Reagent

Yellow solid. Water soluble analog of SMPB. Has been used to

study the rate at which protein is synthesized and transported to

the cell surface. Water soluble. M.W. 458.36 C₁₈H₁₇N₂O₉SNa

Ref: Bangs, J.D., et al. (1986) J. Cell Biol. 103, 255-263.

N-Trifluoroacetyl Imidazole

G.C. Acylation Reagent

Colorless liquid. TFAI gives a smooth and positive reaction

without release of acids into the system to hydrolyze samples.

TFAI will acylate hydroxyl groups, primary and secondary amines.

Purity >97%. M.W. 164.09 CAS 1546-79-8 C₅H₃N₂OF₃

Ref: Seeley, S.D. and Powell, L.E. (1974) Anal. Biochem. 58, 39-46.

0.2M Trimethylanilinium Hydroxide in Methanol

Alkylation Reagent

Colorless solution. TMPAH is a powerful methylating reagent for

quantitive methylation and detection of barbiturates, sedatives

xanthine bases and phenolic alkaloids.

Ref: Brochmann, Hansen and Oke, (1969) J. Pharm. Sci. 58, 370-371.

G.C. Silylation Reagent

Colorless liquid. A potent and selective TMS donor that reacts with

alcohols and phenols but not with amines or amides. Has been

used to derivatize hindered hydroxyl groups and wet sugar samples.

Purity >98%. M.W. 140.26 CAS 18156-74-6 C₆H₁₂N₂Si

Ref: Vanden Heuvel, W.T.A., et al. (1960) Biochem Biophys. Res. Commun. 3, 356.

2-Iminothiolane

Protein Modification Reagent

White solid. Used to introduce sulfhydryl groups onto primary

amines. 2-Iminothiolane is water soluble and reacts at pH7-10.

M.W. 127.63 CAS 4781-83-3 C₄H₇NS^.HCl

Ref: Ghosh, S.S., et al. (1990) Bioconjugate Chem. 1, 71-76.