p-Azidobenzoyl Hydrazide

Photoactivatable, carbohydrate-reactive cross-linker

Off white solid. Reacts with cis-diol containing carbohydrates or

other molecules having been oxidized to form aldehydes.

Photoazide end reacts nonspecifically with molecules upon UV

photolysis. Protect from light.

Purity >90% by HPLC. Soluble in methylene chloride.

M.W. 177.17 C₇H₇ON₅

Ref: O’Shannessy, D.J., et al. (1985) J. Applied Biochem. 7, 347-355.

Succinimidyl-7-amino-4-methylcoumarin-3-acetate

Amino Reactive Probe

Molecular probe that is an amine reactive fluorophore. Off white

to light yellow solid. Protect from light.

Purity >90% by HPLC. M.W. 330.09 C₁₆H₁₄N₂O₆

Ref: Harlow, E., et al. (1988) Antibodies: A Laboratory Manual.

Cold Spring Harbor, New York, p.562.

N-5-Azido-2-nitrobenzoyloxysuccinimide

Amine Reactive, Nitrophenylazide

Phenylazide with a nitro group that shifts the wave length for

photolysis to 320-350 nm. This helps maintain biological

integrity of proteins and nucleic acids. Orange solid. Protect

TLC, one homogeneous spot. M.W. 305.23 C₁₁H₇O₆N₅

Ref: Schafer, H., Chemical Modification of Enzymes, Ed. Eyzaguirre, J.

(1987) pp.45-62.

N-[4-(p-Azidosalicylamido)butyl]-3-(2-pyridyldithio)Propionamide

Sulfhydryl-photoreactive reagent

A cleavable, sulfhydryl reactive photo reactive cross-linker that is

radioiodinatable. Off white solid. Protect from light.

M.W. 446.55 C₁₉H₂₁O₃N₆S₂

Ref: Traut, R.R., et al. Protein function, A Practical Approach.

Edited by T.E. Creighton, IRL Press, 1989, p. 101.

p-Azidophenyl Glyoxal ^. H₂O

Photoreactive and reacts selectively with arginine residues

Reacts wtih amine groups and guanidium side chain of arginine.

Off white to yellow powder. Protect from light.

Ref: Ngo, T.T., et al. (1981) J. Biol. Chem. 256, 11313-11318.

4-(p-Azidosalicylamido) butylamine

Iodinatable and Photoreactive cross-linker

The amine terminal will react with a carboxylic acid in the pressence

of EDC to form an amide bond. Amide product is then ready to

iodinate, then undergo photo-coupling. Off white solid.

Protect from light. M.W. 249.27 C₁₁H₁₅O₂N₅

N,N,Bis-acryloyl cystamine

Reversible cross-linker for polyacrylamide gels

BAC is used as the cross-linking agent in formulations

of polyacrylamide gels. The resultant cross-linked gel

can be dissolved with 2-mercaptoethenol after electro-

phoresis. m.p. 121-123°. M.W. 260.4 C₁₀H₁₆N₂O₂S₂

Ref: Hansen, J.N. (1976) Anal. Biochem. 76, 37-44.

Bis[B-(4-azidosalicylamido)ethyl] disulfide

Cleavable photoreactive reagent that is iodinatable

BASED reacts nonspecifically with proteins. It is useful when no

amines or sulfhydryls are in a location suitable to obtain the

desired conjugate. Protect from light. Off white solid.

Purity >90% by elemental analysis.

M.W. 474.54 C₁₈H₁₈O₄N₈S₂

Boron Trifluoride-methanol complex

Forms methyl esters of organic acids. Purity 13-17% BF₃.

CAS 373-57-9 M.W. 99.85

1 gm 75.00

Zwitterionic Detergent

White solid. Hydoscopic. Non-ionic detergent analog of

CHAPS and CHAPSO with significantly reduced electostatic

interactions that do not interfer in anion exchange chromatography.

Purity >95% by TLC. Soluble in H₂O.

N-[6-(Biotinamido)hexyl]-3^’-(2^’-pyridyldithio) Propionamide

Sulfhydryl Reactive Biotinylation Reagent

Biotinylation occurs between pH 6-9. Pyridyldithiol reacts with

sulfhydryl groups to form a stable disulfide bond. The

pyridine-2-thione leaving group can be measured at 343 nm to

determine the degree of biotinylation. White solid.

Purity >90% by HPLC. M.W. 539.77 C₂₄H₂₇O₃N₅S₃

Ref: Ghebrehiwet, B., et al. (1988) J. Immunol. Methods 110, 251-260.