Carbohydrate Reactive Biotinylation Reagent

Biotinylates immunoglobulins without loss of immunological

activity. Use at pH 4-6 to complete coupling reaction. White powder.

Purity >95% by TCL. CAS 66640-86-6.

M.W. 258.3 C₁₀H₁₈O₂N₄S

Ref: O’Shannessy, D.J., et al. (1984) Immun. Letters 8, 273-277.

Biotinamidocaproyl Hydrazide

Carbohydrate Reactive, Spacer Arm, Biotinylation Reagent

Biotinylates oxidized sugars, glycoproteins with a 6 carbon spacer

between biotin and the ligand, alleviating steric hindrance.

Off white solid. Purity >95%. Soluble in MeOH and H₂O.

M.W. 371.5 C₁₆H₂₉O₃N₅S

Ref: O’Shannessy, D.J. et al. (1987) Anal. Biochem. 163, 204-209.

5-(Biotinamido)Pentylamine

Colorimetric assays for Factor XII Carboxyls

White solid. Use with EDC to couple with carboxylic acid groups.

Water soluble. M.W. 328.48 C₁₄H₂₈O₂N₄S

Ref: Lee, K.N., et al. (1988) Clin. Chem. 34, 906

Easy CMC Determination

Prepare test solutions with varying amounts of detergent.

Add blue ball and observe the amount of blue dye solubilized.

Plot the absorbance of the blue dye at 612 nm on a microwell

reader. Graph the two intersecting lines. The CMC is equal to

the amount of detergent at the point of intersection. One can

"eye ball" the CMC with good accuracy.

Bismaleimido butane

Homobifunctional Reagent

White solid. Used as a bridge between free sulfhydryl

groups of biological molecules. The two maleimido functional

groups permit irreversible cross-linking of sulfhydryl containing

compounds under mild conditions.

M.W. 248.29 C₁₂H₁₂N₂O₄

Bismaleimido hexane

Homobifunctional Reagent

White solid. Used as a bridge between free sulfhydryl

groups of biological molecules. The two maleimido functional

groups permit irreversible cross-linking of sulfhydryl containing

compounds under mild conditions.

M.W. 276.29 C₁₄H₁₆N₂O₄

Bismaleimido propane

Homobifunctional Reagent

White solid. Used as a bridge between free sulfhydryl groups

of biological molecules. The two maleimido functional groups

permit irreversible cross-linking of sulfhydryl containing com-

pounds under mild conditions.

M.W. 234.29 C₁₁H₁₀N₂O₄

(1-biotinamido)-4-[4-(maleimidomethyl) cyclohexane-

carboxamido] butane

Sulfhydryl Reactive Biotinylation Reagent

Biotin maleimido reagent that reacts at a neutral to slightly acidic

pH to form a thiol ether linkage. White solid. Soluble in DMF.

M.W. 561.7 C₂₈H₄₃O₅N₅S

2-(2’-Nitrophenylsulfenyl)-3-methyl-3-bromoindolenine

Cleaves tryptophan residues

Yellow solid. BNPS-Skatole is useful for cleaving tryptophan

residues. The amino acid sequence of cytotoxin mitogillin was

determined with peptide fragments produced by BNPS-Skatole.

Purity : approx. 90%. M.W. 363.2 CAS 27933-36-4

C₁₅H₁₁N₂O₂SBr

Ref: Fernandez-Luna, et al. (1985) Biochem. J. 24, 861-867

N-Succinimidyl-3-(4-hydroxyphenyl) propionate

Iodination Reagent

Reacts with N-terminal amino groups at pH 8.5. Iodinate before

or after coupling to molecule of interest. White solid. M.W. 263.26

Purity: 90%. CAS 3407-95-9 C₁₃H₁₃O₅N

Ref: Bolton, A.E. and Hunter, W.M. (1973) Biochem. J. 133, 529-539.

Sulfosuccinimidyl-3-(4-hyroxyphenyl) propionate

Water Soluble Iodination Reagent

Reacts with N-terminal amino groups at pH 8.5. Iodinate before

or after coupling to molecule of interest. White solid. M.W. 365.30

C₁₃H₁₂O₈NSNa

Ref: Thompson, J.A., et al (1987) Biochem 26, 743-750.

Polyoxyethylene 23 Laurel Ether

Purified Nonionic Detergent

10 % aqueous solution. Useful for the isolation of functional

membrane complexes.Purified to reduce levels of peroxides,

aldehydes and heavy metals. Purity: aldehydes <20 uM,

peroxides <200 uM; Conductivity <50umhos. CAS 9002-92-0.

Ref: Chang, H.W. and Bock, E. (1980) Anal. BioChem. 104, 112-117.

Polyoxyethylene 10 Cetyl Ether

Purified Nonionic Detergent

10% aqueous solution. Useful for the isolation of functional

membrane complexes. Purified to reduce levels of peroxides,

aldehydes and heavy metals. Purity: aldehydes <200 uM,

peroxides <200 uM; Conductivity <50umhos. CAS 9004-95-9.

Ref: Chang, H.W. and Bock, E. (1980) Anal. Biochem. 104, 112-117.

Polyoxyethylene 20 Cetyl Ether

Purified Nonionic Detergent

10% aqueous solution. Useful for the isolation of functional

membrane complexes. Purified to reduce levels of peroxides,

aldehydes and heavy metals. Purity: aldehydes <200uM,

peroxides < 200uM; Conductivity <50 umhos. CAS 9004-95-9.

Ref: Chang, H.W. and Bock, E. (1980) Anal. Biochem. 104, 112-117.

G.C. Silylation Reagent

Forms highly stable TMS derivatives wtih most organic functional

groups under mild reaction conditions. Purity >97%. M.W. 203.43.

CAS 10416-59-8 C₈H₂₁NOSi₂

Ref: Welch, M.J., et al. (1993) J. of Toxicology 17, 389.

Bis-[2-(succinimidooxycarbonyloxy)ethyl] sulfane

Non cleavable, amino reactive, water soluble cross-linker

Off white solid. Base-cleavable, cross-linker useful for studying

receptors and mapping surface polypeptic antigens on

lymphocytes.

M.W. 640.5 C₁₄H₁₆O₁₂N₂S

Ref: Howard, A.D., et al. (1985) J. Biol. Chem. 260, 10833.

Bis(sulfosuccinimidyl) Suberate

Non cleavable, amine reactive, water soluble cross-linker

Off white solid. non-cleavable, membrane- impermeable

cross-linker. Used for conjugating radio-labeled ligands to cell

surface receptors. Soluble in H₂O. Purity >90% by HPLC.

M.W. 572.4. C₁₆H₁₈O₁₄N₂S₂Na₂

Ref: Staros, J.V. (1982) Biochemistry 21, 3950.

100 gm 138.00

G.C. Silylation Reagent

Reacts faster than BSA, due to presence of TFA group.

Biproducts and BSTFA are highly volatile allowing good

seperation of early eluding peaks. Purity >97%.

M.W. 257.4 CAS 25561-30-2 C₈H₁₈ONSi₂F₃

Ref: Wang, W.L., et al. (1994) J. of Chrom. B 660, 279

G.C. Silylation Reagent

Addition of TMCS to BSTFA catalyzes reactions of hindered

functional groups in secondary alcohols and amines.

Purity >97%. CAS 25561-30-2.

Ref: Cone, E.J., et al. (1994) Clinical Chemistry, 40 (7), 1299.

Bis(1,1-trifluoroacetoxy) iodobenzene

Protein Modification Reagent

Used for estimating asparagine and glutamine in peptides and

proteins. Has also been used to determine selenocyanate

and thiocyanate ions in the presence of cyanide.

Ref: Soby, L.M. and Johnson, P. (1981) Anal. Biochem. 113, 149.

4-N-N-Dimethylaminoazobenzene 4-Isothiocyanate

Amino Acid Derivatizing Reagent

Red-orange solid. Purity >90% by TLC. M.W. 282.4

CAS 7612-98-8 C₁₅H₁₄N₄S

Ref: Chang, J.Y. and Creaser, E.H., et al. (1976) Biochem. J. 153, 607.

5-Dimethylaminonapthalene-1-sulfonyl chloride

Fluorometric Detection Reagent

Yellow-orange solid. Yeilds highly fluorescent peptide

derivatives. Purity .95%. M.W. 269.7

CAS605-65-2 C₁₂H₁₂NO₂SCl

Ref: Oray, B., et al. (1983) J. Chromatogr. 270, 253.

100 ml slurry 50% 633.00

Affinity Gel

Detergent removing gel has a very low capacity for protein

and extraordinary high capacity for detergents commonly

employed for solubilization of membrane proteins. The

principle of the gel is a combination of a powerful detergent

binding ligand attached to a small pore matrix which excludes

proteins having a molecular weight greater than 10,000.

Protein/detergent samples in buffer compositions having a

pH from 3 to 10 are efficiently stripped of detergent using

the gel. The presence of at least 100 uM phosphate and/or

200 uM NaCl in sample buffer will greatly enhance detergent

binding to the gel.

p-phenylene diisothiocyanate

Homobifunctional Reagent

Crystalline solid. Used to couple peptides containing lysine

to amino polystyrene resins. Soluble in CHCl₃ and DMF.

M.W. 192.3. CAS 4044-65-9. C₈H₄N₂S₂

Ref: Laursen, R.A., et al. F E B S Lett. 21, 67 (1972).

Dimethyl Adipimidate^.2HCl

Homobifunctional Imidoester Cross-linker

White powder. Used to cross-link outer membrane proteins.

Purity >95% by titation. M.W. 245.1

CAS14620-72-5 C₈H₁₆N₂O₂^.2HCl

Ref: Wang, D. and Moore, S. (1977) Biochemistry 16, 2937-2941.