Dimethyl Pimelimidate^.2HCl

Homobifunctional Imidoester Cross-linker

White solid. Water soluble homobifunctional cross-linker that

reacts rapidly with amines at pH 8-10. The amide bond formed

retains net change character of the protein. Purity >95% by

tritation. M.W. 259.2 CAS 58537-94-3 C₉H₁₈N₂O₂^.2HCl

Ref: Brew, K., et al. (1975) J. Biol. Chem. 250, 1434-1444.

Dimethyl Suberimidate^.2HCl

Homobifunctional Imidoester Cross-linker

White solid. Water soluble homobifunctional cross-linker that

reacts rapidly with amines at pH 8-10. The amide bond formed

retains net change character of the protein. Purity >95% by

tritation. M.W. 273.2 CAS 34490-86-3 C₁₀H₂₀N₂O₂^.2HCl

Ref: Dower, S.K., et al. (1981) Biochemistry 20, 6326.

1,4-Di[3’-(2’-pyridyldithio)propionamido]butane

Sulfydryl Reactive Homobifunctional Reagent

White solid. Reacts with sulfhydryls to form a mixed disulfide.

The disulfide can be readily cleaved with a reducing reagent.

Purity >90% by HPLC. M.W. 482.7 C₂₀H₂₆N₂O₂S₄

Dithiobis (succinimidyl propionate)

Cleavable Homobifunctional Reagent

White solid. A thiol cleavable cross-lilnking reagent. 50 mMDTT,

100 mM mercaptoethanol or 1% sodium borohydride can be used

to reduce the disulfide bond. M.W. 404.4 CAS 57757-57-0

C₁₄H₁₆N₂O₈S₂

Ref: Tavers, R.C., et al. (1982) J. Biol. Chem. 257, 10708.

Disuccinimidyl Suberate

Homobifunctional Reagent

White solid. Noncleavable, amine reactive homobifunctional

cross-linker. Has been used for conjugating radiolabeled ligand

to cell surface receptor. Purtiy >95% by TLC. M.W. 368.3

CAS 68528-80-3 C₁₆H₂₀N₂O₈

Ref: Sawyer, S.T., et al. (1987) PNAS 84, 3690-3694.

Dissuccinimidyl Tartarate

Cleavable Homobifunctional Reagent

White solid. A cleavable, homobifunctional reagent. Can be

cleaved with .015M sodium periodate or other oxidizing

reagents. M.W. 344.24 C₁₂H₁₂N₂O₁₀

Ref: Smith, R.J., et al. (1978) Biochemistry 17, 3719-3723.

Dimethyl 3,3-dithiobispropionimidate^.2HCl

Cleavable Imidoester Cross-linker

White solid. Penetrates intact human erythrocytes and cross-links

many of the membrane proteins to hemoglobin. M.W. 309.3

CAS 38285-78-8 C₈H₁₆N₂O₂S₂^.2HCl

Ref: Wang, K. and Richards, F.M. (1974) J. Biol. Chem. 249, 8005-8018.

Dithiobis Phenylazide

Photoreactive Homobifunctional Reagent

Photoreactive reagent that is sensitive to subtle changes in

membrane structures as a function of pH. Reversible by disulfide

reduction wtih mercaptoethanol. Light sensitive.

M.W. 300.4 CAS 37434-06-3 C₁₂H₈N₆S₂

Ref: Mikkelsen, R.B. and Wallach, D.F.H. (1976) J. Biol. Chem. 251, 7413.

3,3 Dithiobis (sulfosuccinimidyl propionate)

Homobifunctional Reagent

Off white solid. Thiol cleavable cross-linker. The disulfide bond

can be cleaved with 50 mM DTT, 100 mM mercaptoethanol or

1% NaBH₄. Has been used to immobilize proteins onto supports

containing amino groups. Purity >95% by HPLC. M.W. 608.5

C₁₄H₁₂N₂O₁₄S₄Na₂

Ref: Lee, W.T. and Conrad, D.H. (1985) J. Immunol. 134, 518-525.

Ethyleneglycol-bis-(succinimidylsuccinate)

Homobifuctional Reagent

White solid. Used for cross-linking and reversible immobilization

of proteins through their amine groups. This reagent has been

used to study structure-function relationships. Readily cleaved at

pH 8.5 with hydroxylamine for 3-5 hours at 37°C. Purity >80% by

HPLC. Soluble in DMSO. M.W. 456.4 CAS 70539-42-3

C₁₈H₂₀N₂O₁₂.

Ref: Conn, P.M., et al. (1982) Nature 296, 653-655.

Maleimido caproic acid hydrazide^.HCl

Heterobifunctional Reagent

White solid. Has an oxidized carbohydrate specific hydrazide

and a sulfhydryl reactive maleimide. Spacer arm accommodates

a wide range of molecular cross-linking demands.

M.W. 261.8 C₁₀H₁₅N₃O₃^.HCl

Maleimido caproic acid N-hydroxysuccinimide ester

Heterobifunctional Reagent

White solid. Sulfhydryl and amine reactive reagent used for

enzyme immunoassay. M.W. 308.3 CAS 55750-63-5

C₁₄H₁₆N₂O₆

Ref: Fujiwara, K., et al. (1981) J. Immunol. Meth., 45, 195.

Ethylene Glycol 50% Cryoprotectant Grade

Purified Cryoprotectant

Clear colorless solution. Purified to remove aldehydes, peroxides,

iron and UV-absorbing impurities. Used to stablize native enzyme

structures and activity.

Conductivity<1.5 umhos, Aldehyde concentration <100 uM,

Peroxide concentration <200 uM. CAS 107-21-1

Ref: Washabaugh, M. and Collins, K. (1983) Anal. Biochem. 134, 144.

4- Fluoro-3-nitrophenyl Azide

Photoaffinity Label

Straw colored needles. Photoaffinity labeling compound for

labeling of antibodies with aryl nitrene as the reactive group.

Light sensitive. M.W. 182.1 CAS 28166-06-5 C₆H₃N₄O₂F

Ref: Fleet, G. and Porter, R., Nature 224, 511-512.

Glycerol 66% Cryoprotectant Grade

Purified Cryoprotectant

Clear colorless solution. Purified to remove aldehydes, peroxides,

iron and UV absorbing impurities. Used to stabilize native enzyme

structure and activity. M.W. 92.09 CAS 56-81-5 C₃H₈O₃

Ref: Washabaugh, M. and Collins, K. (1983) Anal. Biochem. 134, 144.

Maleimidobutyric acid N^.hydoxysuccinimide Ester

Heterobifunctional Reagent

White solid. Useful for preparation of conjugates for EIA procedures

or for immobilizing enzymes on solid supports. Has a low potential

for illiciting an immune response. M.W. 280.2 C₁₂H₁₂N₂O₆

Ref: Rusin, K.M., et al. (1992) Biosens. Bioelectron. 7, 367.

G.C. Acylation Reagent

Colorless liquid. Ideal for preparing fluoroacyl derivitives for GC/MS.

HFAA derivitives are the most sensitive to ECD. Purity >99%.

M.W. 410.06 CAS 336-59-4 C₈O₃F₁₄

Ref: Jones, J.B., et al. (1993) J. of Analytical Toxicology 17, 447.

Heptafluorobutyryl Imidazole

G.C. Acylation Reagent

Colorless liquid. Undergoes fast reactives with phenols,

alcohols and amines to form derivatives that are suitable

for ECD. The imidazole by product is not acidic so no

decomposition or corrosion occurs on the column.

Purity >99%. M.W. 264.1 C₅H₃N₂OF₃

Ref: Miyazaki, H., et al. (1973) Anal. Chem. 45 (7) 1164.