1,1,1,3,3,3-Hexamethyldisilazane

G.C. Silylation Reagent

Colorless liquid. HMDS is a weak TMS doner that is used for

silylation of carbohydrates. Purity >97%. M.W. 161.4

CAS 999-97-3 C₆H₁₉NSi₂

Ref: Swealey, C.C., et al (1963) JACS 85, 2495-2507.

p-Hydroxyphenyl Glyoxal

Protein Modification Reagent

Solid. Reactsspecifically with arginine residues at pH 7-9 at

25°C. Reaction can be monitored at 340 nm at pH 9.

M.W. 168.15 C₈H₆O₃^.H₂O

Ref: Yamasaki, R.B., et al, (1980) Anal. Biochem. 109, 32-40.

3-(4-hydroxphenyl) propionic acid hydrazide^.HCl

Iodination Reagent

White solid. A hydrazide analog of Bolton-Hunter Reagent, for

coupling to oxidized carbohydrates.

M.W. 216.65 C₉H₁₂N₂O₂^.HCl

N-Hydroxysuccinimidyl-4-azidobenzoate

Photoaffinity Cross-linker

White solid. HSAB is ideal for a wide range of photoaffinity

applications. Light sensitive. Purity 95%.

M.W. 260.2 CAS 53053-08-0 C₁₁H₈N₄O₄

Ref: Galardy, R.E., et al., (1974) J. Biol. Chem. 249, 3518.

Biological Buffer

White solid. Excellant for buffer use in pH range 6.2-7.8.

Purity >99% by tritation. Heavy metals <1 ppm.

Soluble in H₂O. M.W. 68.1. CAS 288-32-4

C₃H₄N₂

Ref: Merk Index 12, 4948.

N-Iodoacetyl-N-biotinylhexylenediamine

Biotinylation Reagent

Off white solid. Reacts specifically with thiol groups at pH 7.5-8.5

to form a stable thiol-ether bond. Reagent is not water soluble

so a small amount of DMSO or DMF should be added to the ligand

containing buffer. Soluble in DMSO, DMF.

M.W. 510.42 C₁₈H₃₁N₄O₃SI

Ref: Suton, K., et al. (1984) J. Mol. Bio. 178, 323-339.

Protein Modification Reagent

White solid. One step modification reagent for cysteine. Under

alkylation conditions the sulfhydryl group is ethylated and the

trifluoroacetyl group is lost to give an amine. M.W. 267.0

CAS 67680-56-2 C₄H₅NOF₃I

Ref: Schwartz, W.E., Smith, P.K., and Royer G.P. et al(1980) Anal. Biochem. 106, 43-48.

Protein Modification Reagent

White solid. Protein cleavage at the tryptophenyl bond have been

acheived with this reagent while tyrosine and histidine are unaffected.

M.W. 264.0 CAS 304-91-6 C₇H₅O₃I

Ref: Mahoney, W.C., et al. (1981) Biochemistry 20, 443-448.

Enzyme Test Reagent

White solid. IPTG is an inducer of B-D-galactoside. IPTG is used

to induce the expression of cloned genes that are linked to the lac

promotoer, and for selection of lac Y mutants. Purity >90% by TLC.

Soluble in alcohol and H₂O. M.W. 282.4 CAS 367-93-1

C₉H₁₈O₅S

Ref: (1981) J. Immunol. 127, 1652.

Iodination Reagent

Yellow solid. Amine reactive iodination reagent.

M.W. 178.97 C₇H₆NO₂SB

1-Fluoro-2,4-dinitrophenyl-5-l-alanine-amide

Detection Reagent

Yellow solid. Margey’s derivatizes optical isomers in less than

2 hours. Derivatives can be detected by UV at 340 nm with

picomole sensitivity. M.W. 272.2 CAS 95713-52-3

C₉H₉N₄O₅F

Ref: Marfey, P., (1984) Carlsberg Res. Commun. 49, 591.

N-Methyl-bis-(heptafluorobutyramide)

G.C. Acylation Reagent

Colorless liquid. Used in dual derivatization with a silylation

reagent (MSTFA) eliminatng any acidty or oxidation step. Procedes

optimal seperation of amphetamine, methamphetamine,

phenylpropanolamine and ephedrine which also elute in this order.

M.W. 423.1 C₉H₃NO₂F₁₄

Ref: Clouette, J., et al. Abstract 48, SOFT, Utah 1997.

m-Maleimidobenzoyl-N-hydroxysuccinimide ester

Heterobifunctional Cross-linker

White solid. A cross-linker that is useful for conjugation of

enzymes to antibodies, haptens to carrier proteins and toxins

to antibodies. Purity >95% by TLC. Soluble in DMSO and Chloroform.

M.W. 314.2 CAS 58626-38-3 C₁₅H₁₀N₂O₆

Ref: Kitagawa, T. and Aikawa, T.J., (1976) Biochem. 79, 233.

G.C. Acylation Reagent

Colorless liquid. Reacts quickly under mild conditions with primary

and secondary amines. Reacts slower with alcohols, phenols and

thiols. Is also useful in the analysis of sugars. Purity >98%.

M.W. 223. 08 CAS 685-27-8 C₄H₃NO₂F₆

Ref: Donike, M. (1973) J. of Chem. 78, 273.

Maleimidopropionic Acid Hydrazide

Heterobifunctional Reagent

White solid. Has an oxidized carbohydrate specific hydrazide

and a sulfhydryl reactive maleimide. Spacer arm is shorter than

EMCH. M.W. 219.63 C₇H₉N₃O₃^.HCl

Maleimidopropionic acid N-hydroxysuccinimide ester

Heterobifunctional Reagent

White solid. Sulfhydryl and amine reactive reagent used for

enzyme immunoassay. Shorter spacer arm than EMCS.

M.W. 266.2 CAS 55750-62-4 C₁₁H₁₀N₂O₆

Ref: Kitagawa, T., Enz. Immunoassay, 81, (1981).

N-Methyl-N-trimethylsilyl-heptafluorobutyramide

G.C. Silylation Reagent

Colorless liquid. This reagent is a TMS derivatizing reagent that

has an increased fluorine content that causes less SiO₂ fouling

of FID detectors. M.W. 299.26 C₈H₁₂NOF₇Si

Ref: Donike, M. (1976) Chem. Abstr. 85, 103518.

G.C. Silylation Reagent

Colorless liquid. Useful in the analysis of volatile trace materials.

The most volatile of the TMS-acetamides. Purity >97%.

M.W. 199.25 CAS 24589-78-4 C₆H₁₂NOSiF₃

Ref: Gleispuch, H. (1974) j. Chrom. 91, 407-412.

with 1% TMCS

G.C. Silylation Reagent

Colorless liquid. The most volatile TMS-acetamide available.

TMCS helps catalyze reaction. Forms volatile and stable derivatives

for GC/MS.

M.W. 199.25 CAS 24589-78-4 C₆H₁₂NOSiF₃

Ref: Doniker, M. (1969) J. Chromatogr. 42, 103-104.

G.C. Silylation Reagent

Colorless liquid. MTBSTFA derivatives are more stable to hydrolysis

than their corresponding TMS derivatives. Reacts with active

hydrogens to form stable t-BDMS derivatives.

M.W. 241.33 CAS 77377-52-7 C₉H₁₈NOSiF₃

Ref: Harkey, M.R., et al. (1991) J. of Anal. Tox. 15, 260.